GOGO熟女少妇大尺度,99RE热这里只有精品视频,亚洲码国产精品高潮在线,精品人人搡人妻人人玩A片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

簡(jiǎn)要描述:COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1819

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human

Tissue  colon

Product Format  frozen

Morphology  rounded and refractile

Culture Properties  mixed, adherent and suspension

Biosafety Level  1

Disease  Dukes' type C, colorectal adenocarcinoma

Age  55 years

Gender  female

Ethnicity  Caucasian

Storage Conditions

Karyotype  modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Images

Clinical Data  55 years

Caucasian

female

Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Tumorigenic  Yes

Effects  Yes, in nude mice

Comments  The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

3.Add appropriate aliquots of the cell suspension to new culture vessels.

4.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Isoenzymes  ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Name of Depositor  GE Moore

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲AV无码乱码国产麻豆| 久久久无码一区二区三区| 国产亚洲精品一区二区在线观看 | 人体欣赏SHOWYBEAUTY| 亚洲国产精华液2020| 夜夜躁天天躁很躁| 亚洲18色成人网站WWW| 国产精品夜间视频香蕉| 极品人妻洗澡后被朋友玩| 亚洲乱码一区AV春药高潮| 无码国产精品一区二区免费16| 天天摸夜夜摸摸到高潮| 疯狂做受XXXX高潮按摩| 国产精品白丝JK白祙喷水视频 | 色偷偷伊人大杳蕉综合网 | 亚洲另类欧美综合久久图片区| 亚洲成AV人片在线观看无码| 被老外添嫩苞添高潮NP| 无码免费视频AAAAAAAA片| 免费看美女被靠到爽的视频 | 国产精品久久久久乳精品爆| 成人性做爰AAA片免费看曹查理| 国产男女猛烈无遮挡免费网站| 亚洲日韩精品无码专区网址| 少妇被躁爽到高潮无码| 无码狠狠躁久久久久久久| 欧美做受又硬又粗又大视频| 免费播放欧美毛片欧美AAAAA| AV无码人妻中文字幕| 最近中文字幕免费MV视频7| 国产熟女高潮精品视频AV| 特黄a级毛片免费视频| 国产做A爰片久久毛片A片白丝| 岳肥肉紧嫩嫩伦69| 翘臀后进娇喘呻吟的熟妇| 高中陪读房间的呻吟声| 中文在线А天堂中文在线新版| 极品少妇HDXX麻豆HDXX| АⅤ资源天堂资源库在线| 外国四个黑人RAPPER组合| 人妻放荡乱绿帽H文|